| |
Gérard Ailhaud and a team of laboratory experts
and clinicians describe in step-by-step detail the major techniques
needed for the study of adipose tissue and cells. Drawn from both
in vivo and in vitro studies, these readily reproducible methods
cover a broad range of techniques, including the choice of adipose
tissue depot and of morphological techniques for work on both
brown adipose tissue (BAT) and white adipose tissue (WAT). Major
treatment is accorded to the isolation, subcellular fractionation,
and transfection of low density adipocytes as well as metabolic
aspects of nutrient uptake and assays of nutrient and ion fluxes.
Innovative and highly practical, Adipose Tissue Protocols offers
endocrinologists, physiologists, cell biologists, and pharmacologists
a gold-standard collection of proven methods for effective research
on adipose tissue from the nutritional to the physiological and
the molecular levels.
|
| |
Preface
Contributors
1. Choosing an Adipose Tissue Depot for Sampling: Factors in Selection
and Depot Specificity
2. Morphologic Techniques for the Study of Brown Adipose Tissue
and White Adipose Tissue
3. Confocal Microscopy of Adipocytes
4. Cellularity Measurements
5. Subcellular Fractionation of Adipocytes and 3T3-L1 Cells
6. Quantification of Lipid-Related mRNAs by Reverse Transcription-Competitive
Polymerase Chain Reaction in Human White Adipose Tissue Biopsies
7. Glycogen Synthase Activity in Adipose Tissue: Methods for Freeze-Clamping
and Assay
8. Assays of Lipolytic Enzymes
9. Assays of Lipogenic Enzymes
10. Assays of Adrenergic Receptors: Including Lipolysis and Binding
Measurements
11. Transfection of Adipocytes and Preparation of Nuclear Extracts
12. Assay of Membrane Transport of Long-Chain Fatty Acids by Adipocytes
13. Assays of Glucose Entry, Glucose Transporter Amount, and Translocation
14. Methods to Study Phosphorylation and Activation of the Hormone-Sensitive
Adipocyte Phosphodiesterase Type 3B in Rat Adipocytes
15. Measurements of Glucose Conversion to its Metabolites
16. Metabolite and Ion Fluxes and Ion Channels in Brown and White
Adipocytes
17. Culture of Adipose Tissue and Isolated Adipocytes
18. Cultures of Adipose Precursor Cells from Brown Adipose Tissue
and of Clonal Brown-Adipocyte-Like Cell Lines
19. Cultures of Adipose Precursor Cells and Cells of Clonal Lines
from Animal White Adipose Tissue
20. Cultures of Human Adipose Precursor Cells
21. Measurements of Peptide and Nonpeptide Secretory Products
from Adipocytes
22. Assessment of White Adipose Tissue Metabolism by Measurement
of Arteriovenous Differences
23. Measurement of Adipose Tissue Blood Flow
24. Respiratory and Thermogenic Capacities of Cells and Mitochondria
from Brown and White Adipose Tissue
25. Measurements of White Adipose Tissue Metabolism by the Microdialysis
Technique
26. Immunoassay Measurements of Leptin
Index
|
